含有硅烷和二苯并環辛炔的PEG DBCO-PEG10000-Silane

產品名稱：DBCO-PEG-Silane

中文名稱：二苯并環辛炔-聚乙二醇-硅烷

PEG分子量：1k，2k，3.4k，5k，10k其他分子量可定制

存儲條件：≤-4℃低溫干燥保存

產品外觀: 固體或黏稠液體，取決于分子量

常用溶劑：溶于大部分有機溶劑，如：DCM、DMF、DMSO、THF等等。在水中有很好的溶解性

DBCO用于無銅點擊化學。在沒有銅催化劑的情況下，與疊氮化合物的反應非常迅速。這種反應是非常迅速，溫和，和生物相容性。

銀催化條件下，DBCO可以快速的酰化氨基官能團；對三種包含20種天然氨基酸的多肽底物研究發現，DBCO酰化反應高選擇性地發生在Lys的氨基上，而不影響其他氨基酸。同時，銀催化DBCO酰化反應可以**地修飾糖肽、BSA蛋白、糖蛋白RNase B 和IgG抗體等，表現出較好的底物適用性。此外，銀催化酰化反應可以實現Ph.D.12商業噬菌體庫的修飾且不影響噬菌體侵染活力，說明該方法具有應用于活生物體系中的潛力。

在反應緩沖液中制備含疊氮化物的樣品。將DBCO共軛物添加到含疊氮化物的樣品中。建議：在豐度試劑的1.5-3.0摩爾當量中添加1摩爾當量的限制試劑。將反應在室溫下于4攝氏度溫育2-4小時或2-12小時。現在可以將反應純化。

DBCO is used for copper free click chemistry. Without copper catalyst, the reaction with azide compound is very rapid. This reaction is very rapid, mild, and biocompatible.

Under silver catalysis, DBCO can quickly acylate amino groups; The study of three peptide substrates containing 20 natural amino acids found that the DBCO acylation reaction occurred on the amino acid of Lys with high selectivity, without affecting other amino acids. At the same time, silver catalyzed DBCO acylation reaction can efficiently modify glycopeptides, BSA proteins, glycoprotein RNase B and IgG antibodies, showing good substrate applicability. In addition, silver catalyzed acylation can achieve the modification of commercial phage library of Ph.D.12 without affecting the infection activity of phages, indicating that this method has potential application in living biological systems.

The sample containing azide was prepared in the reaction buffer. DBCO conjugate was added to the sample containing azide. It is recommended to add a limiting reagent of 1 mole equivalent to the 1.5-3.0 mole equivalent of the abundance reagent. The reaction was incubated at 4 ℃ at room temperature for 2-4 hours or 2-12 hours. The reaction can now be purified.

DBCO-GPC二苯基環辛炔-甘磷酸膽堿

DBCO-PFP二苯基環辛炔-五氟苯酚

DBCO-Benzaldehyde二苯基環辛炔-苯甲醛

DBCO-Mesylate 二苯基環辛炔-甲磺酸酯

DBCO-TPP二苯基環辛炔-磷酸三苯酯

DBCO-Phenyl二苯基環辛炔-苯基

DBCO-acid二苯基環辛炔-磷酸

DBCO-GA二苯基環辛炔修飾甘草次酸

DBCO-Cyclodextrin二苯基環辛炔偶聯環糊精

DBCO-Dopamine 二苯基環辛炔修飾多巴胺

DBCO-Ferrocene二苯基環辛炔修飾二茂鐵

用途:科研

純度:≥95%

保存時間: 1年

產地:西安

供應商：西安齊岳創科生物科技有限公司

注意事項:僅用于科研不用于人體